Ten years after the first American Pie movie, three new hapless virgins discover the Bible hidden in the school library at East Great Falls High. Unfortunately for them, the book is ruined, and with incomplete advice, the Bible leads them on a hilarious journey to lose their virginity.

Rebecca and Michael decide to take a weekend getaway and drive into the isolated countryside, they arrive at their cottage unscathed but Rebecca can’t help but feel like someone is watching.