When Mystery, Inc. are guests of honor at the grand opening of the Coolsville Museum of Criminology, a masked villain shows up and creates havoc before stealing the costumes of the gang’s most notorious villains…Could it be that their nemesis, mad scientist Jonathan Jacobo has returned and is trying to recreate their deadliest foes?

After 52 years of solving mysteries, the Scooby gang reunite at Warner Bros. Studios to reminisce about their favorite cases and how they were filmed. But it turns out that the back lot may have its OWN monster problem. Fred, Daphne, Velma, Shaggy, and Scooby-Doo investigate this mystery while celebrating the legacy of the franchise.

Scooby-Doo! Mystery Incorporated is the eleventh incarnation of Hanna-Barbera’s Scooby-Doo animated series, and the first incarnation not to be first-run on Saturday mornings. The series is produced by Warner Bros. Animation and Cartoon Network and premiered in the United States on Cartoon Network on April 5, 2010, with the next twelve episodes continuing, and the first episode re-airing, on July 12, 2010. The series concluded on April 5, 2013 with two seasons and fifty-two episodes, with a total of twenty-six episodes per season.

Mystery Incorporated returns to the early days of Scooby and the gang, when they are still solving mysteries in their home town, though it makes many references to previous incarnations of the franchise, not least among them many cases and creatures from the original Scooby-Doo, Where Are You!. Episode by episode, the series takes a tongue-in-cheek approach to the classic Scooby-Doo formula, with increasingly outlandish technology, skills and scenarios making up each villain’s story, and a different spin on the famous “meddling kids” quote at the end of every episode. Contrasting sharply with this, however, are two elements that have never been used in a Scooby-Doo series before: a serial format with an ongoing story arc featuring many dark plot elements that are treated with near-total seriousness, and ongoing relationship drama between the characters.